Insights into the Influences of Carboxymethyl-β-Cyclodextrin on DNA Formulations Characteristics and Gene Transfection Efficiency.
| Autor: | Elsana H; University of Sunderland, Sunderland, United Kingdom., Mysina S; University of Sunderland, Sunderland, United Kingdom., Elkordy EA; Faculty of Medicine, Tanta University, Tanta, Egypt., Carr-Wilkinson J; University of Sunderland, Sunderland, United Kingdom., Elkordy AA; University of Sunderland, Sunderland, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Current drug delivery [Curr Drug Deliv] 2018; Vol. 15 (6), pp. 867-878. |
| DOI: | 10.2174/1567201815666180226115503 |
| Abstrakt: | Background: Gene therapy is an expanding field and it can treat genetic and acquired diseases. Objective: It was found that formulations with DNA: CM-β-CD (Carboxymethyl-beta-cyclodextrin): Pluronic-F127 1:3:3 and 1:3 DNA: CM-β-CD are the most stable formulations indicating high incorporation of DNA within CM-β -CD. Method: Gel electrophoresis revealed DNA with low CM-β -CD concentration has formed a more stable complex. Samples 1:3 DNA: CM-β-CD and 1:3:3 DNA: CM-β-CD: Pluronic-127 show no DNA fragment, suggesting good condensation of DNA inside cyclodextrin cavity. Results: This was confirmed by fluorescence data where fluorescence intensity was reduced for samples DNA: CM-β-CD 1:3. Overall, the findings showed that Carboxymethyl-beta-cyclodextrin (as a novel non-viral gene vector) was able to provide condensation and protection to the DNA, with and without Pluronic-F127, at low concentration. Conclusion: pDNA/CM-β-CD complex has not only shown to be able to transfect COS 7 and SHSY5Y cell lines, but it gives a higher transfection efficiency than that produced by the TransIT-LT1 commercial transfection reagent. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.) |
| Databáze: | MEDLINE |
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