Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity 18 F-FDG injection.

Autor: Kirov AS; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Fanchon LM; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Seiter D; Grove City College, Grove City, PA, 16127, USA., Czmielewski C; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Russell J; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Dogan S; Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Carlin S; Department of Radiology, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104, USA., Pinker-Domenig K; Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Yorke E; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Schmidtlein CR; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Boyko V; Molecular Cytology Core Facility, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Fujisawa S; Molecular Cytology Core Facility, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Manova-Todorova K; Molecular Cytology Core Facility, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Zanzonico P; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Dauer L; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Deasy JO; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Humm JL; Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA., Solomon S; Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.
Jazyk: angličtina
Zdroj: Medical physics [Med Phys] 2018 May; Vol. 45 (5), pp. 2179-2185. Date of Electronic Publication: 2018 Mar 23.
DOI: 10.1002/mp.12836
Abstrakt: Purpose: Genomic profiling of biopsied tissue is the basis for precision cancer therapy. However, biopsied materials may not contain sufficient amounts of tumor deoxyribonucleonic acid needed for the analysis. We propose a method to determine the adequacy of specimens for performing genomic profiling by quantifying their metabolic activity.
Methods: We estimated the average density of tumor cells in biopsy specimens needed to successfully perform genomic analysis following the Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT) protocol from the minimum amount of deoxyribonucleonic acid needed and the volume of tissue typically used for analysis. The average 18 F-FDG uptake per cell was assessed by incubating HT-29 adenocarcinoma tumor cells in 18 F-FDG containing solution and then measuring their activity with a scintillation well counter. Consequently, we evaluated the response of two devices around the minimum expected activities which would indicate genomic profiling adequacy of biopsy specimens obtained under 18 F-FDG PET/CT guidance. Surrogate samples obtained using 18G core needle biopsies of gels containing either 18 F-FDG-loaded cells in the expected concentrations or the corresponding activity were measured using autoradiography and a scintillation well counter. Autoradiography was performed using a CCD-based device with real-time image display as well as with digital autoradiography imaging plates following a 30-min off-line protocol for specimen activity determination against previously established calibration.
Results: Cell incubation experiments and estimates obtained from quantitative autoradiography of biopsy specimens (QABS) indicate that specimens acquired under 18 F-FDG PET/CT guidance that contained the minimum amount of cells needed for genomic profiling would have an average activity concentration in the range of about 3 to about 9 kBq/mL. When exposed to specimens with similar activity concentration, both a CCD-based autoradiography device and a scintillation well counter produced signals with sufficient signal-to-background ratio for specimen genomic adequacy identification in less than 10 min, which is short enough to allow procedure guidance.
Conclusion: Scintillation well counter measurements and CCD-based autoradiography have adequate sensitivity to detect the tumor burden needed for genomic profiling during 18 F-FDG PET/CT-guided 18G core needle biopsies of liver adenocarcinoma metastases.
(© 2018 American Association of Physicists in Medicine.)
Databáze: MEDLINE
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