A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress.
| Autor: | Denanglaire S; Laboratoire d'Immunobiologie, Université Libre de Bruxelles-IBMM, Gosselies, Belgium., Pirnay T; Laboratoire d'Immunobiologie, Université Libre de Bruxelles-IBMM, Gosselies, Belgium., Leo O; Laboratoire d'Immunobiologie, Université Libre de Bruxelles-IBMM, Gosselies, Belgium., Andris F; Laboratoire d'Immunobiologie, Université Libre de Bruxelles-IBMM, Gosselies, Belgium. fandris@ulb.ac.be. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1732, pp. 465-476. |
| DOI: | 10.1007/978-1-4939-7598-3_29 |
| Abstrakt: | Distinct lymphocyte subpopulations display discrete metabolic profiles and are differently affected by metabolic resource variations, making the analysis of lymphocyte survival in a complex tissue in response to metabolic stress highly challenging. Here we describe a flow cytometry-based method allowing simultaneous cell identification and viable cell counting in mixed lymphocyte populations without extensive cell subset purification procedures. The example provided herein illustrates the role of AMPK in T lymphocyte survival in response to the mitochondrial poison oligomycin. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|