Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina.

Autor: Bouffard S; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Dambroise E; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Brombin A; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Lempereur S; Tefor Core Facility, TEFOR Infrastructure, NeuroPSI, CNRS, Gif-sur-Yvette, France; Université Paris-Est, LIGM, ESIEE, Noisy-le-Grand, France., Hatin I; Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 400, 91400 Orsay, France., Simion M; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Corre R; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Bourrat F; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France., Joly JS; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France; Tefor Core Facility, TEFOR Infrastructure, NeuroPSI, CNRS, Gif-sur-Yvette, France., Jamen F; INRA CASBAH Group, Neurosciences Paris-Saclay Institute, CNRS, Université Paris-Saclay, Université Paris-Sud, Gif-sur-Yvette, France. Electronic address: francoise.jamen@cnrs.fr.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2018 May 01; Vol. 437 (1), pp. 1-16. Date of Electronic Publication: 2018 Feb 23.
DOI: 10.1016/j.ydbio.2018.02.006
Abstrakt: Fibrillarin (Fbl) is a highly conserved protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. In cellular models, FBL was shown to play an important role in tumorigenesis and stem cell differentiation. We used the zebrafish as an in vivo model to study Fbl function during embryonic development. We show here that the optic tectum and the eye are severely affected by Fbl depletion whereas ventral regions of the brain are less impacted. The morphogenesis defects are associated with impaired neural differentiation and massive apoptosis. Polysome gradient experiments show that fbl mutant larvae display defects in ribosome biogenesis and activity. Strikingly, flow cytometry analyses revealed different S-phase profiles between wild-type and mutant cells, suggesting a defect in S-phase progression.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: