Induction profiles of mRNA of toll like receptors and cytokines in chickens pre-exposed to low pathogenic avian influenza H9N2 virus followed by challenge with highly pathogenic avian influenza H5N1 virus.

Autor: Dash SK; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Kumar M; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India. Electronic address: mjoshi@hsadl.nic.in., Kataria JM; ICAR-Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India., Nagarajan S; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Tosh C; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Murugkar HV; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Rajukumar K; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Kalaiyarasu S; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Senthil Kumar D; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India., Kulkarni DD; ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh, 462022, India.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2018 Apr; Vol. 117, pp. 200-205. Date of Electronic Publication: 2018 Feb 21.
DOI: 10.1016/j.micpath.2018.02.041
Abstrakt: Herein, the induction of TLRs and cytokines in chickens pre-exposed to low pathogenic avian influenza H9N2 virus followed by challenge with highly pathogenic avian influenza (HPAI) H5N1 virus was studied. Four groups (1-4) of chickens inoculated with 10 6 EID 50 of H9N2 virus were challenged with 10 6 EID 50 of H5N1 virus on days 1, 3, 7 and 14 post H9N2 inoculation, respectively. In groups (1-4) TLRs and cytokines induction was studied in chicken PBMCs on day 3 post H5N1 challenge. In H5N1 control group TLRs (1, 2, 5 and 7) cytokines (IFNα, IFNβ, IFNγ, IL1β, IL2, IL4, IL8 and TGF β3) were down regulated. In group 1 down regulation of cytokines and TLRs was similar to H5N1 control birds. Down regulation of TLRs and cytokines in H5N1 control and group 1 resulted death of all the chickens. In group 2, up-regulation of TLRs (3, 7 and 15) and induction of TNFα, IFNα, IFNβ, IFNγ aided virus clearance leading to survival of all the chickens. In group 3 significant up-regulation of TLRs (3, 4 and 15) and significant induction of cytokines (IFNγ, TNFα, IL1β, IL4, IL6, IL8, IL10 and TGF β3) was detected. In group 4 significant up-regulation of TLRs (2, 3, 7 and 15) and significant induction of cytokines (IFNγ, TNFα, IL1β, IL2, IL6, IL8 and IL10) was detected. In groups 3 and 4 simultaneous and significant induction of pro-inflammatory, antiviral and anti-inflammatory cytokine resulted cytokine dysregulation leading to death of (2/6) and (3/6) chickens respectively. Hence, the study revealed TLRs and cytokines role in modulating the H5N1 infection outcome in chickens pre-exposed to H9N2 virus.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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