Evaluation of CD307a expression patterns during normal B-cell maturation and in B-cell malignancies by flow cytometry.

Autor: Auat M; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Cardoso CC; Clinical Analysis Division, University Hospital of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Santos-Pirath IM; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.; Clinical Analysis Division, University Hospital of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Rudolf-Oliveira RCM; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Matiollo C; Clinical Analysis Division, University Hospital of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Lange BG; Clinical Analysis Department of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., da Silva JP; Clinical Analysis Department of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Dametto GC; Hematology and Hemotherapy Center of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Pirolli MM; Hematology and Hemotherapy Center of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Colombo MDHP; Hematology and Hemotherapy Center of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Santos-Silva MC; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.; Clinical Analysis Division, University Hospital of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.; Clinical Analysis Department of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Jazyk: angličtina
Zdroj: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2018 Jul; Vol. 94 (4), pp. 588-595. Date of Electronic Publication: 2018 Mar 09.
DOI: 10.1002/cyto.b.21631
Abstrakt: Background: Flow cytometric immunophenotyping is deemed a fundamental tool for the diagnosis of B-cell neoplasms. Currently, the investigation of novel immunophenotypic markers has gained importance, as they can assist in the precise subclassification of B-cell malignancies by flow cytometry. Therefore, the purpose of the present study was to evaluate the expression of CD307a during normal B-cell maturation and in B-cell malignancies as well as to investigate its potential role in the differential diagnosis of these entities.
Methods: CD307a expression was assessed by flow cytometry in normal precursor and mature B cells and in 115 samples collected from patients diagnosed with precursor and mature B-cell neoplasms. CD307a expression was compared between neoplastic and normal B cells.
Results: B-acute lymphoblastic leukemia cases exhibited minimal expression of CD307a, displaying a similar expression pattern to that of normal B-cell precursors. Mantle cell lymphoma (MCL) cases showed the lowest levels of CD307a among mature B-cell neoplasms. CD307a expression was statistically lower in MCL cases than in chronic B lymphocytic leukemia (CLL) and marginal zone lymphoma (MZL) cases. No statistical differences were observed between CD307a expression in neoplastic and normal plasma cells.
Conclusion: These results indicate that the assessment of CD307a expression by flow cytometry could be helpful to distinguish CLL from MCL, and the latter from MZL. Although these results are not entirely conclusive, they provide a basis for further studies in a larger cohort of patients. © 2018 International Clinical Cytometry Society.
(© 2018 International Clinical Cytometry Society.)
Databáze: MEDLINE
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