Genetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder, Paralichthys lethostigma.

Autor: O'Leary SJ; Department of Life Sciences, Marine Genomics Laboratory, Texas A&M University Corpus Christi, 6300 Ocean Drive, Unit 5869, Corpus Christi, TX, 78412, USA. shannon.j.oleary@gmail.com., Hollenbeck CM; Scottish Oceans Institute, University of St. Andrews, East Sands, St. Andrews, Fife, KY16 8LB, UK., Vega RR; Texas Parks and Wildlife Department, CCA Marine Development Center, 4300 Waldron Road, Corpus Christi, TX, 78418, USA., Gold JR; Department of Life Sciences, Marine Genomics Laboratory, Texas A&M University Corpus Christi, 6300 Ocean Drive, Unit 5869, Corpus Christi, TX, 78412, USA., Portnoy DS; Department of Life Sciences, Marine Genomics Laboratory, Texas A&M University Corpus Christi, 6300 Ocean Drive, Unit 5869, Corpus Christi, TX, 78412, USA.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2018 Feb 23; Vol. 19 (1), pp. 163. Date of Electronic Publication: 2018 Feb 23.
DOI: 10.1186/s12864-018-4541-0
Abstrakt: Background: Southern flounder, Paralichthys lethostigma, historically support a substantial fishery along the Atlantic and Gulf coasts of the southern United States. Low year-class strengths over the past few years in the western Gulf of Mexico have raised concern that spawning stocks may be overfished. Current management of the resource includes releasing hatchery-raised juveniles to restock bays and estuaries; additionally, there is a growing interest in the potential for commercial aquaculture of the species. Currently, genomic resources for southern flounder do not exist. Here, we used two hatchery-reared families and double-digest, restriction-site-associated DNA (ddRAD) sequencing to create a reduced-representation genomic library consisting of several thousand single nucleotide polymorphisms (SNPs) located throughout the genome.
Results: The relative position of each SNP-containing locus was determined to create a high-density genetic map spanning the 24 linkage groups of the southern flounder genome. The consensus map was used to identify regions of shared synteny between southern flounder and seven other fish species for which genome assemblies are available. Finally, syntenic blocks were used to localize genes identified from transcripts in European flounder as potentially being involved in ecotoxicological and osmoregulatory responses, as well as QTLs associated with growth and disease resistance in Japanese flounder, on the southern flounder linkage map.
Conclusions: The information provided by the linkage map will enrich restoration efforts by providing a foundation for interpreting spatial genetic variation within the species, ultimately furthering an understanding of the adaptive potential and resilience of southern flounder to future changes in local environmental conditions. Further, the map will facilitate the use of genetic markers to enhance restoration and commercial aquaculture.
Databáze: MEDLINE
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