Citrobacter freundii impairs the phosphoryl transfer network in the gills of Rhamdia quelen: Impairment of bioenergetics homeostasis.

Autor: Baldissera MD; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: mdbaldissera@mail.ufsm.br., Souza CF; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Junior GB; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Moreira KLS; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., da Veiga ML; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., da Rocha MIUM; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., Baldisserotto B; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: bernardo@smail.ufsm.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2018 Apr; Vol. 117, pp. 157-161. Date of Electronic Publication: 2018 Feb 19.
DOI: 10.1016/j.micpath.2018.02.040
Abstrakt: The precise coupling of spatially separated intracellular adenosine triphosphate (ATP)-producing and ATP-consuming, catalyzed by creatine kinase (CK), adenylate kinase (AK), and pyruvate kinase (PK), is a critical process in the bioenergetics of tissues with high energy demand, such as the branchial tissue. The effects of Citrobacter freundii infection on gills remain poorly understood, limited only to histopathological studies. Thus, the aim of this study was to evaluate whether experimental infection by C. freundii impairs the enzymes of the phosphoryl transfer network in gills of silver catfish (Rhamdia quelen). The CK (cytosolic and mitochondrial) and AK activities decreased in infected compared to uninfected animals, while the PK activity did not differ between groups. The gill histopathology of infected animals revealed extensive degeneration with fusion and necrosis of secondary lamellae, detachment of superficial epithelium, aneurysm, vessel congestion and inflammatory process. Based on these evidences, the inhibition and absence of an efficient communication between CK compartments caused the impairment of the branchial bioenergetics homeostasis, which was not compensated by the augmentation on branchial AK activity in an attempt to restore energy homeostasis. In summary, these alterations contribute to disease pathogenesis linked to branchial tissue in animals infected with C. freundii.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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