Spectroscopic and Hydrodynamic Characterisation of DNA-Linked Gold Nanoparticle Dimers in Solution using Two-Photon Photoluminescence.

Autor: Midelet J; Physics and Astronomy, University of Southampton, Southampton, SO17 1BJ, United Kingdom., El-Sagheer AH; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom., Brown T; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom., Kanaras AG; Physics and Astronomy, University of Southampton, Southampton, SO17 1BJ, United Kingdom., Débarre A; Lab. Aimé-Cotton and PPSM, École Normale Supérieure de Cachan, CNRS, 61 Av. du Président Wilson, 94235, Cachan, France., Werts MHV; Univ Rennes, CNRS, SATIE-UMR8029, 35000, Rennes, France.; École Normale Supérieure de Rennes, Av. R. Schuman, Campus de Ker Lann, 35170, Bruz, France.
Jazyk: angličtina
Zdroj: Chemphyschem : a European journal of chemical physics and physical chemistry [Chemphyschem] 2018 Apr 05; Vol. 19 (7), pp. 827-836. Date of Electronic Publication: 2018 Feb 21.
DOI: 10.1002/cphc.201701228
Abstrakt: Two-photon photoluminescence (TPPL) emission spectra of DNA-gold nanoparticle (AuNP) monoconjugates and the corresponding DNA-linked AuNP dimers are obtained by photon time-of-flight spectroscopy. This technique is combined with two-photon photoluminescence fluctuation correlation spectroscopy (TPPL-FCS) to simultaneously monitor the optical and hydrodynamic behaviour of these nano-assemblies in solution, with single-particle sensitivity and microsecond temporal resolution. In this study, the AuNPs have an average core diameter of 12 nm, which renders their dark-field plasmonic light scattering too weak for single-particle imaging. Moreover, as a result of the lack of plasmonic coupling in the dimers, the optical extinction, scattering and photoluminescence spectra of the DNA-AuNP complexes are not sufficiently different to distinguish between monomers and dimers. The use of TPPL-FCS successfully addresses these bottlenecks and enables the distinction between AuNP monomers and AuNP dimers in solution by measurement of their hydrodynamic rotational and translational diffusion.
(© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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