| Autor: |
Bezerra NM; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., Moura GHF; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., de Araújo HN Jr; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil. helio.noberto@outlook.com., Bezerra FSB; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., de Paiva KAR; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., de Freitas Mendonça Costa KM; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., Costa WP; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., Medeiros DAS; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil., Batista JS; Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil. |
| Abstrakt: |
The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25 × 10 5 trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed. Semen samples were collected from goats by electroejaculation on the 7th, 14th and 21st days post-infection (dpi). The recombinant DNA-encoding gene encoding the L-like-specific gene for T. vivax. The infection was characterized by increased rectal temperature, high parasitemia and significant reduction of hematocrit values. Results for T. vivax DNA detection using TviCatL-PCR were positive in all semen samples from the infected group collected on 7th, 14th and 21st dpi. The presence of T. vivax DNA in 7th dpi suggests the early invasion of the parasite in the reproductive organs. Also, the finding of T. vivax DNA in all periods analyzed may suggest the continued elimination of the parasite in the semen, which may increase the chances of sexual transmission. Thus, T. vivax DNA is recorded for the first time in the semen of infected goats. Thus, these data are of great importance, since the detection of the T. vivax genetic material in the semen may point to the possibility that the parasite may be transmitted through the sexual pathway. |
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