Performance Characteristics of Different Anti-Double-Stranded DNA Antibody Assays in the Monitoring of Systemic Lupus Erythematosus.
| Autor: | Mahler M; Inova Diagnostics Inc., San Diego, CA, USA., Bentow C; Inova Diagnostics Inc., San Diego, CA, USA., O'Malley T; Exagen Diagnostics, Vista, CA, USA., Ibarra C; Exagen Diagnostics, Vista, CA, USA., Conklin J; Exagen Diagnostics, Vista, CA, USA., Aure MAR; Inova Diagnostics Inc., San Diego, CA, USA., Dervieux T; Exagen Diagnostics, Vista, CA, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of immunology research [J Immunol Res] 2017; Vol. 2017, pp. 1720902. Date of Electronic Publication: 2017 Dec 31. |
| DOI: | 10.1155/2017/1720902 |
| Abstrakt: | Objective: We sought to evaluate different anti-double-stranded DNA assays for their performance characteristics in monitoring disease activity fluctuations in systemic lupus erythematosus (SLE). Methods: 36 active SLE patients were followed monthly. At each study visit (total n = 371), blood was collected and disease activity was scored using the SELENA-SLEDAI (excluding anti-dsDNA or complement components) and by a physician's global assessment (PGA). Four anti-dsDNA tests were compared. Linear mixed-effects models with random intercept and fixed slopes were used to evaluate the relationship between the longitudinal fluctuations of disease activity and anti-dsDNA titers. Results: At enrollment, positivity for QUANTA Lite and high-avidity anti-dsDNA assay was both 64% and significantly lower than anti-dsDNA positivity by QUANTA Flash (83%) and CLIFT (96%). Linear mixed-effects modeling indicated that the change in clinical SELENA-SLEDAI scores was associated with the titers of all anti-dsDNA with QUANTA Flash yielding the highest marginal R 2 (0.15; p < 0.01). QUANTA Flash was the only anti-dsDNA assay significantly associated with the change in PGA (marginal R 2 = 0.05; p < 0.01). Conclusion: These data indicate that anti-dsDNA antibodies determined by QUANTA Flash have a value in monitoring SLE disease activity. |
| Databáze: | MEDLINE |
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