[Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma].

Autor:	Osmolovskiy AA; Biological Faculty of Lomonosov Moscow State University, Moscow, Russia., Orekhova AV; Biological Faculty of Lomonosov Moscow State University, Moscow, Russia; Faculty of Fundamental Medicine of Lomonosov Moscow State University, Moscow, Russia., Kreyer VG; Biological Faculty of Lomonosov Moscow State University, Moscow, Russia., Baranova NA; Biological Faculty of Lomonosov Moscow State University, Moscow, Russia., Egorov NS; International Biotechnology Center of Lomonosov Moscow State University, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2018 Jan; Vol. 64 (1), pp. 115-118.
DOI:	10.18097/PBMC20186401115
Abstrakt:	It was shown that the activator activity of protein C, determined in normal plasma using Aspergillus ochraceus protease, is comparable with the activity of commercial protease analogue from the South American copperhead venom (ProtacÒ). It was found that protease of A. ochraceus can be used to determine protein C in plasma with its reduced content similar to ProtacÒ. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may make it a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.
Databáze:	MEDLINE
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