[Construction and characterization of a recombinant mutant homolog of the CheW protein from Thermotoga petrophila RKU-1].

Autor:	Grishin DV; Institute of Biomedical Chemistry, Moscow, Russia., Zhdanov DD; Institute of Biomedical Chemistry, Moscow, Russia., Gladilina JA; Institute of Biomedical Chemistry, Moscow, Russia., Pokrovsky VS; Institute of Biomedical Chemistry, Moscow, Russia., Podobed OV; Institute of Biomedical Chemistry, Moscow, Russia., Pokrovskaya MV; Institute of Biomedical Chemistry, Moscow, Russia., Aleksandrova SS; Institute of Biomedical Chemistry, Moscow, Russia., Milyushkina AL; Institute of Biomedical Chemistry, Moscow, Russia., Vigovskiy MA; Institute of Biomedical Chemistry, Moscow, Russia., Sokolov NN; Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2018 Jan; Vol. 64 (1), pp. 53-60.
DOI:	10.18097/PBMC20186401053
Abstrakt:	In the work a recombinant chemotaxis protein CheW from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homolog (TpeCheW-mut) were created. It was shown that, despite the low homology with CheW prototypes from intestinal bacteria, these proteins didn't cause metabolic overload and were well expressed by cells of E. coli laboratory strains. We have discovered a broad spectrum of industrial valuable properties of the TpeCheW-mut protein such as stability in a wide range of temperatures and pH, high expression level, solubility and possibility of the application of a simple low-stage purification methodology with the use of preliminary heat treatment. Possible directions of the scientific and industrial application of this protein were claimed.
Databáze:	MEDLINE
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