[Use of DNA-aptamers for enrichment of low abundant proteins in cellular extracts for quntitative detection by selected reaction monitoring].

Autor:	Ptitsyn KG; Institute of Biomedical Chemistry, Moscow, Russia., Novikova SE; Institute of Biomedical Chemistry, Moscow, Russia., Kiseleva YY; Russian Scientific Center of Roentgenoradiology, Moscow, Russia., Moysa AA; Institute of Biomedical Chemistry, Moscow, Russia., Kurbatov LK; Institute of Biomedical Chemistry, Moscow, Russia., Farafonova TE; Institute of Biomedical Chemistry, Moscow, Russia., Radko SP; Institute of Biomedical Chemistry, Moscow, Russia., Zgoda VG; Institute of Biomedical Chemistry, Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2018 Jan; Vol. 64 (1), pp. 5-9.
DOI:	10.18097/PBMC20186401005
Abstrakt:	The relationship between the amount of a target protein in a complex biological sample and its amount measured by selected reaction monitoring (SRM) mass spectrometry upon the affinity enrichment of target protein with aptamers immobilized on a solid phase was studied. Human thrombin added in known concentrations to cellular extracts derived from bacterial cells was used as model target protein. It has been demonstrated that the affinity enrichment of thrombin in cellular extracts by means of the thrombin-binding aptamer immobilized on the surface of magnetic microbeads results in an approximately 10-fold increase of the concentration of target protein and a 100-fold decrease of the low limit of a target protein concentration range where its quantitative detection by SRM is possible without an interference from other peptides present in a tryptic digest.
Databáze:	MEDLINE
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