Revealing the inhibitory potential of Yersinia enterocolitica on cysteine proteases of the papain family.

Autor: Kędzior M; Department of Physical Chemistry of Microorganisms, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland. Electronic address: mateusz.kedzior@uwr.edu.pl., Pawlak A; Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland., Seredyński R; Department of Physical Chemistry of Microorganisms, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland; Department of Physiology, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland., Bania J; Department of Food Hygiene and Consumer Health Protection, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland., Platt-Samoraj A; Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland., Czemplik M; Department of Physical Chemistry of Microorganisms, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland., Klausa E; Regional Centre of Transfusion Medicine and Blood Bank, Czerwonego Krzyża 5, 50-345 Wrocław, Poland., Bugla-Płoskońska G; Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland., Gutowicz J; Department of Physical Chemistry of Microorganisms, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2018 Mar; Vol. 207, pp. 211-225. Date of Electronic Publication: 2017 Dec 08.
DOI: 10.1016/j.micres.2017.12.005
Abstrakt: Cysteine proteases of the papain family, including mammalian cathepsins, play important physiological roles, however, their excessive activity may contribute to the development of various pathologies. Therefore, cysteine cathepsin inhibitors are being considered as promising drugs to treat cathepsin-driven diseases. Diverse saprophytic and parasitic microbes produce such inhibitors, which target the host's proteases playing pivotal roles in immune responses, thus leading to the survival of microbes within their host. Yersinia enterocolitica is a Gram-negative zoopathogenic coccobacillus, which has developed several mechanisms to evade the host's immune system. Nevertheless, the bacterium has not yet been shown to produce any cysteine protease inhibitors. Here we demonstrate that Y. enterocolitica strains of different bioserotypes and genotypes synthesize papain and human cathepsin L inhibitors, but not bovine cathepsin B inhibitors. By employing fluorimetry and zymography, the cell-surface inhibitors were shown to associate peripherally with the outer membrane, while the inhibitors present in cell-free extracts proved to: interact reversibly with their target enzymes, exhibit thermolability and stability in a range of pH values (5-9), and have high molecular weights. Batch affinity chromatography on papain-agarose resin was then undertaken to isolate putative inhibitors of cysteine proteases from the bacterial extract. The isolated 18 kDa protein was identified by LC-MS/MS as the periplasmic chaperone Skp. The Skp-containing eluate inhibited the activity of cysteine cathepsins produced by human dermal fibroblasts. The homologous Skp protein was also isolated from the extract of Escherichia coli. Our results point to a possible new biological role of the bacterial chaperone Skp.
(Copyright © 2017 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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