Bisphenol A alters oocyte maturation by prematurely closing gap junctions in the cumulus cell-oocyte complex.

Autor: Acuña-Hernández DG; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico., Arreola-Mendoza L; Departamento de Biociencias e Ingenieria CIIEMAD, del Instituto Politécnico Nacional, México City, Mexico., Santacruz-Márquez R; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico., García-Zepeda SP; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico., Parra-Forero LY; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico., Olivares-Reyes JA; Departamento de Bioquímica, Cinvestav, IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico., Hernández-Ochoa I; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), IPN 2508, Col. San Pedro Zacatenco, México City 07360, Mexico. Electronic address: mihernandez@cinvestav.mx.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2018 Apr 01; Vol. 344, pp. 13-22. Date of Electronic Publication: 2018 Feb 16.
DOI: 10.1016/j.taap.2018.02.011
Abstrakt: In ovarian follicles, cumulus cells communicate with the oocyte through gap junction intercellular communication (GJIC), to nurture the oocyte and control its meiosis arrest and division. Bisphenol A (BPA) is a monomer found in polycarbonate-made containers that can induce functional alterations, including impaired oocyte meiotic division and reduced molecule transfer in GJIC. However, how BPA alters oocyte meiotic division is unclear. We investigated whether BPA effects on oocyte meiotic division were correlated with reduced transfer in GJIC. Cumulus cell-oocyte complexes (COCs) isolated from mouse preovulatory follicles were cultured with 0, 0.22, 2.2, 22, 220, and 2200 nM BPA for 2 h. An additional 16-h incubation with epidermal growth factor (EGF) was performed to promote the occurrence of meiotic resumption and progression to metaphase II. Without EGF stimulus, BPA treatment increased the percentage of oocytes undergoing meiotic resumption, decreased GJIC in the COCs, and did not modify GJIC gene (Cx43 and Cx37) and protein (CX43) expression. Following EGF stimulus, BPA increased the percentage of oocytes that remained at the anaphase and telophase stages, and decreased the percentage of oocytes reaching the metaphase II stage. Concomitantly, BPA reduced the expansion of cumulus cells. Carbenoxolone (a GJIC inhibitor) and 6-diazo-5-oxo-l-norleucine (a cumulus cell-expansion inhibitor) exerted effects on meiotic division similar to those exerted by BPA. These data suggest that BPA accelerates meiotic progression, leading to impaired prophase I-to-metaphase II transition, and that this adverse effect is correlated with reduced bidirectional communication in the COC.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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