Effect of multiple low-dose PGF 2 α injections on the mature corpus luteum in non-pregnant bitches.

Autor: Ucar EH; Adnan Menderes University Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Aydin, Turkey., Cetin H; Adnan Menderes University Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Aydin, Turkey., Atli MO; Dicle University Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Diyarbakir, Turkey. Electronic address: moatli@hotmail.com.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2018 Jun; Vol. 113, pp. 34-43. Date of Electronic Publication: 2018 Feb 22.
DOI: 10.1016/j.theriogenology.2018.01.018
Abstrakt: This study investigated molecular regulation in the canine corpus luteums/corpora lutea (CL) following multiple low-dose prostaglandin F 2 alpha (PGF 2 α) injections in non-pregnant bitches around 30-35 days after ovulation. The CL were obtained by ovariohysterectomy 1 h after the last PGF 2 α injection. The subjects were divided into the following groups: control (no PGF 2 α injection, n = 4), one PGF 2 α injection (injection at 0 h, 1PGF, n = 4), two PGF 2 α injection (injection at 0 and 8 h, 2PGF, n = 4), and three PGF 2 α injection (injection at 0, 8 and 24 h, 3PGF, n = 4). In the 1PGF group, the steady-state mRNA levels of an immediate early gene (NR4A1) and immune system-related genes (MCP-1 and IL-8) increased. NR4A1 was localized in luteal and endothelial cells. In contrast, MCP-1 was localized in the luteal tissue between the luteal and endothelial cells. LHCGR, CYP11A1, and StAR mRNA expression decreased after the second PGF 2 α injection. FASLG increased only after the third PGF 2 α injection. The mRNA levels of PTGFR, PGT, and PTGS2 decreased as the number of PGF 2 α injections increased. Immunohistochemistry showed a decrease in StAR protein density as the number of PGF 2 α injections increased. BAX and CASP3 mRNA expression levels were similar among the groups. Serum progesterone (P 4 ) levels decreased dramatically after the PGF 2 α injections but were still higher than the basal level at the end of the study. In conclusion, repeated low-dose PGF 2 α injections could induce luteolytic mechanisms in the CL of non-pregnant bitches. Furthermore, it can be concluded that, in non-pregnant bitches, some aspects of the molecular regulation of luteolysis in the CL are similar to some aspects of such regulation in other domestic animals.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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