Cadherin-26 (CDH26) regulates airway epithelial cell cytoskeletal structure and polarity.

Autor:	Lachowicz-Scroggins ME; 1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 USA.; 2Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, San Francisco, CA 94143 USA., Gordon ED; 2Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, San Francisco, CA 94143 USA., Wesolowska-Andersen A; 3Center for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206 USA., Jackson ND; 3Center for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206 USA., MacLeod HJ; Johns Hopkins Bloomberg School of Public Health, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Baltimore, MD 21205 USA., Sharp LZ; 1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 USA., Sun M; 1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 USA., Seibold MA; 3Center for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206 USA.; 5Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045 USA., Fahy JV; 1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 USA.; 2Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, San Francisco, CA 94143 USA.
Jazyk:	angličtina
Zdroj:	Cell discovery [Cell Discov] 2018 Feb 13; Vol. 4, pp. 7. Date of Electronic Publication: 2018 Feb 13 (Print Publication: 2018).
DOI:	10.1038/s41421-017-0006-x
Abstrakt:	Polarization of the airway epithelial cells (AECs) in the airway lumen is critical to the proper function of the mucociliary escalator and maintenance of lung health, but the cellular requirements for polarization of AECs are poorly understood. Using human AECs and cell lines, we demonstrate that cadherin-26 (CDH26) is abundantly expressed in differentiated AECs, localizes to the cell apices near ciliary membranes, and has functional cadherin domains with homotypic binding. We find a unique and non-redundant role for CDH26, previously uncharacterized in AECs, in regulation of cell-cell contact and cell integrity through maintaining cytoskeletal structures. Overexpression of CDH26 in cells with a fibroblastoid phenotype increases contact inhibition and promotes monolayer formation and cortical actin structures. CDH26 expression is also important for localization of planar cell polarity proteins. Knockdown of CDH26 in AECs results in loss of cortical actin and disruption of CRB3 and other proteins associated with apical polarity. Together, our findings uncover previously unrecognized functions for CDH26 in the maintenance of actin cytoskeleton and apicobasal polarity of AECs. Competing Interests: The authors declare that they have no conflict of interest.
Databáze:	MEDLINE
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