Quantitative comparisons between optical coherence tomography angiography and matched histology in the human eye.

Autor: An D; Centre for Ophthalmology and Visual Science, University of Western Australia, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia. Electronic address: dong.an@health.wa.gov.au., Balaratnasingam C; Centre for Ophthalmology and Visual Science, University of Western Australia, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Sir Charles Gairdner Hospital, Hospital Ave, Nedlands, Western Australia, 6009, Australia. Electronic address: balaratnasingam@gmail.com., Heisler M; School of Engineering Science, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada. Electronic address: mheisler@sfu.ca., Francke A; School of Engineering Science, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada. Electronic address: afrancke@sfu.ca., Ju M; School of Engineering Science, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada. Electronic address: mjju@sfu.ca., McAllister IL; Centre for Ophthalmology and Visual Science, University of Western Australia, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Royal Perth Hospital, 197 Wellington St, Perth, Western Australia, 6000, Australia. Electronic address: ianmacallister@lei.org.au., Sarunic M; School of Engineering Science, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada. Electronic address: msarunic@sfu.ca., Yu DY; Centre for Ophthalmology and Visual Science, University of Western Australia, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia; Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia, 6009, Australia. Electronic address: dyyu@lei.org.au.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2018 May; Vol. 170, pp. 13-19. Date of Electronic Publication: 2018 Feb 12.
DOI: 10.1016/j.exer.2018.02.006
Abstrakt: The aim was to quantitatively compare retinal vascular detail as seen on optical coherence tomography angiography (OCTA) and matched histology in the human eye. 13 normal human donor eyes were used. The central retinal artery was cannulated after which human packed red blood cells were perfused through the retinal vasculature. Retinal vessels were imaged using a custom-built OCTA device during red blood cell perfusion. The eye was subsequently perfused with endothelial cell antibodies and the flat-mounted retina studied histologically using a confocal scanning laser microscope. Qualitative and quantitative comparisons of retinal vascular information as seen on OCTA and histology from the same region of interest were performed. Gradable OCTA images were acquired from 4 of 13 eyes with mean postmortem-to-OCTA imaging time of 4.5 ± 1.3 h 23 pairs of OCTA-histology matched images were evaluated. The retinal arteries and veins had similar pixel intensity on OCTA images. The diameter of retinal veins was significantly greater than its paired artery on OCTA (P < 0.001). The density of vascular structures on OCTA (40.2% ± 10.1%) was significantly less than matched histology (52.1% ± 9.3%, P < 0.001). Mean capillary diameter on OCTA (10.2 ± 2.4 μm) was significantly greater than histology (8.2 ± 2.4 μm; P < 0.001). This is the first study to directly compare OCTA against histology from the same human eye. OCTA visualizes many of the vascular structures in the human retinal circulation but does not exactly match what is seen on histologic examination.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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