| Autor: |
Giraldo A; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading; a.giraldoramirez@reading.ac.uk., Talavera López J; Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad de Murcia; talavera@um.es., Fernandez-Del-Palacio MJ; Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad de Murcia., García-Nicolás O; Institute of Virology and Immunology (IVI); Departamento de Anatomía y Anatomía Comparada, Facultad de Veterinaria, Universidad de Murcia., Seva J; Departamento de Anatomía y Anatomía Comparada, Facultad de Veterinaria, Universidad de Murcia., Brooks G; Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading., Moraleda JM; Unidad de Trasplante Hematopoyético y Terapia Celular, Departamento de Hematología, Hospital Universitario Virgen de la Arrixaca, IMIB, Universidad de Murcia. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2018 Jan 21 (131). Date of Electronic Publication: 2018 Jan 21. |
| DOI: |
10.3791/56699 |
| Abstrakt: |
Cell and gene therapy are exciting and promising strategies for the purpose of cardiac regeneration in the setting of heart failure with reduced ejection fraction (HFrEF). Before they can be considered for use, and implemented in humans, extensive preclinical studies are required in large animal models to evaluate the safety, efficacy, and fate of the injectate (e.g., stem cells) once delivered into the myocardium. Small rodent models offer advantages (e.g., cost effectiveness, amenability for genetic manipulation); however, given inherent limitations of these models, the findings in these rarely translate into the clinic. Conversely, large animal models such as rabbits, have advantages (e.g., similar cardiac electrophysiology compared to humans and other large animals), whilst retaining a good cost-effective balance. Here, we demonstrate how to perform a percutaneous contrast echocardiography-guided intramyocardial injection (IMI) technique, which is minimally invasive, safe, well tolerated, and very effective in the targeted delivery of injectates, including cells, into several locations within the myocardium of a rabbit model. For the implementation of this technique, we also have taken advantage of a widely available clinical echocardiography system. After putting in practice the protocol described here, a researcher with basic ultrasound knowledge will become competent in the performance of this versatile and minimally invasive technique for routine use in experiments, aimed at hypothesis testing of the capabilities of cardiac regenerative therapeutics in the rabbit model. Once competency is achieved, the whole procedure can be performed within 25 min after anaesthetizing the rabbit. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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