Characterizing the clinical isolates of dermatophytes in Hamadan city, Central west of Iran, using PCR-RLFP method.

Autor: Farokhipor S; Department of Microbiology, Islamic Azad University, Arak Branch, Arak, Iran., Ghiasian SA; Medical Parasitology and Mycology Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Psoriasis Research Center, Department of Dermatology, Farshchian Hospital, Hamadan University of Medical Sciences, Hamadan, Iran., Nazeri H; Mycology Laboratory, Farshchian Hospital, Hamadan University of Medical Sciences, Hamadan, Iran., Kord M; Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran., Didehdar M; Infectious Diseases Research Center (IDRC), Department of Medical Parasitology and Mycology, Arak University of Medical Sciences, Arak, Iran. Electronic address: didehdar_m@Arakmu.ac.ir.
Jazyk: angličtina
Zdroj: Journal de mycologie medicale [J Mycol Med] 2018 Mar; Vol. 28 (1), pp. 101-105. Date of Electronic Publication: 2018 Feb 11.
DOI: 10.1016/j.mycmed.2017.11.009
Abstrakt: Objective: Dermatophytosis is one of the most common mycotic infections, which considered as a public health problem in the major of countries. This study evaluated the molecular epidemiology of dermatophytosis in patients referred to Farshchian hospital in Hamadan city with PCR-RFLP method.
Materials and Methods: Four hundred and five specimens from clinically suspected patients of dermatophytosis were collected and analyzed by direct microscopic and culture. The isolates were identified by PCR-RFLP method using the MvaI restriction enzyme.
Results: Of the 405 specimens, 88 specimens were positive in direct examination and culture. Among the patients, 64.8% were males and35.2% females. Tinea pedis (31.8%) was the most common type of dermatophytosis followed by tinea corporis (22.7%), tinea cruris (20.5%), tinea capitis (10.2%), tinea manuum (5.7%), tinea faciei (4.6%) and tinea unguium (4.6%). Trichophyton interdigitale (36.4%) was the most common isolate followed by Trichophyton rubrum (27.3%), Epidermophyton floccosum (17%), Trichophyton tonsurans (11.4%), Microsporum canis (4.5%), Microsporum gypseum (2.3%) and Trichophyton benhamiae (1.1%).
Conclusion: Our finding showed that the anthropophilic dermatophyte species causing dermatophytosis are increasing, and molecular methods are reliable assays for accurse identification of dermatophyte species in epidemiological studies.
(Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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