Decreased expression of TERT correlated with postnatal cochlear development and proliferation reduction of cochlear progenitor cells.

Autor: Song YL; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Tian KY; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Mi WJ; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Ding ZJ; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Qiu Y; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Chen FQ; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Zha DJ; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China., Qiu JH; Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
Jazyk: angličtina
Zdroj: Molecular medicine reports [Mol Med Rep] 2018 Apr; Vol. 17 (4), pp. 6077-6083. Date of Electronic Publication: 2018 Feb 06.
DOI: 10.3892/mmr.2018.8565
Abstrakt: Cochlear progenitor cells are considered as one of the best candidates for hair cell regeneration, thus, the regulation of cochlear progenitor cell proliferation has become a focus in this field. Several genes expressed in the inner ear during postnatal development have been demonstrated to be involved in maintaining the proliferative potential of progenitor cells, but the mechanism for regulating the proliferation and differentiation of cochlear progenitor cells remains poorly understood. Telomerase reverse transcriptase (TERT) has rate limiting telomerase activity and the overexpression of TERT has been shown to promote cell proliferation in series of cell lines. The aim of the present study was to evaluate the expression of TERT in the postnatal development of the cochlea and progenitor cells. The results demonstrated that TERT was expressed in the basilar membranes during the first postnatal week. In vitro, TERT expression in progenitor cells reached a maximum at day 4 after culture and decreased as the culture time prolonged or the cell passage number increased. These results led us to hypothesize that TERT may be involved in the development of the cochlea and in maintaining the proliferation ability of progenitor cells.
Databáze: MEDLINE
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