Ellagitannin HeT obtained from strawberry leaves is oxidized by bacterial membranes and inhibits the respiratory chain.

Autor: Martos GG; INSIBIO (CONICET-UNT) and Instituto de Química Biológica San Miguel de Tucuman Argentina., Mamani A; INSIBIO (CONICET-UNT) and Instituto de Química Biológica San Miguel de Tucuman Argentina., Filippone MP; Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC) Tucumán Argentina., Abate PO; INQUINOA (UNT-CONICET) Facultad de Bioquímica Química y Farmacia Universidad Nacional de Tucumán Argentina., Katz NE; INQUINOA (UNT-CONICET) Facultad de Bioquímica Química y Farmacia Universidad Nacional de Tucumán Argentina., Castagnaro AP; Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC) Tucumán Argentina., Díaz Ricci JC; INSIBIO (CONICET-UNT) and Instituto de Química Biológica San Miguel de Tucuman Argentina.
Jazyk: angličtina
Zdroj: FEBS open bio [FEBS Open Bio] 2018 Jan 04; Vol. 8 (2), pp. 211-218. Date of Electronic Publication: 2018 Jan 04 (Print Publication: 2018).
DOI: 10.1002/2211-5463.12361
Abstrakt: Plant secondary metabolism produces a variety of tannins that have a wide range of biological activities, including activation of plant defenses and antimicrobial, anti-inflammatory and antitumoral effects. The ellagitannin HeT (1- O -galloyl-2,3;4,6-bis-hexahydroxydiphenoyl-β-d-glucopyranose) from strawberry leaves elicits a strong plant defense response, and exhibits antimicrobial activity associated to the inhibition of the oxygen consumption, but its mechanism of action is unknown. In this paper we investigate the influence of HeT on bacterial cell membrane integrity and its effect on respiration. A β-galactosidase unmasking experiment showed that HeT does not disrupt membrane integrity. Raman spectroscopy analysis revealed that HeT strongly interacts with the cell membrane. Spectrochemical analysis indicated that HeT is oxidized in contact with bacterial cell membranes, and functional studies showed that HeT inhibits oxygen consumption, NADH and MTT reduction. These results provide evidence that HeT inhibits the respiratory chain.
Databáze: MEDLINE
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