Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis.

Autor: Biswal AK; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Atmodjo MA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Li M; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA., Baxter HL; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA., Yoo CG; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA., Pu Y; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA., Lee YC; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Noble Research Institute, Ardmore, Oklahoma, USA., Mazarei M; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA., Black IM; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA., Zhang JY; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Noble Research Institute, Ardmore, Oklahoma, USA., Ramanna H; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Noble Research Institute, Ardmore, Oklahoma, USA., Bray AL; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA., King ZR; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA., LaFayette PR; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA., Pattathil S; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Donohoe BS; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Mohanty SS; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Ryno D; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Yee K; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Thompson OA; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Rodriguez M Jr; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Dumitrache A; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Natzke J; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Winkeler K; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; ArborGen, Inc., Ridgeville, South Carolina, USA., Collins C; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; ArborGen, Inc., Ridgeville, South Carolina, USA., Yang X; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Tan L; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Sykes RW; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Gjersing EL; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Ziebell A; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Turner GB; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Decker SR; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Hahn MG; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Department of Plant Biology, University of Georgia, Athens, Georgia, USA., Davison BH; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Udvardi MK; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Noble Research Institute, Ardmore, Oklahoma, USA., Mielenz JR; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA., Davis MF; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; National Renewable Energy Laboratory, Golden, Colorado, USA., Nelson RS; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Noble Research Institute, Ardmore, Oklahoma, USA., Parrott WA; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA., Ragauskas AJ; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA.; Department of Chemical and Biomolecular Engineering & Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, Tennessee, USA., Neal Stewart C Jr; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.; Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA., Mohnen D; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2018 Mar; Vol. 36 (3), pp. 249-257. Date of Electronic Publication: 2018 Feb 12.
DOI: 10.1038/nbt.4067
Abstrakt: Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. We engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an α-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.
Databáze: MEDLINE
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