Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8 + T Cell Cross-Priming.

Autor:	van Dinther D; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Veninga H; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Iborra S; Immunobiology Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain., Borg EGF; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Hoogterp L; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Olesek K; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Beijer MR; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Schetters STT; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Kalay H; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Garcia-Vallejo JJ; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Franken KL; Department of Immunohematology and Bloodtransfusion, LUMC, Leiden, the Netherlands., Cham LB; Institute of Immunology, Medical Faculty, University Duisburg-Essen, 45122 Essen, Germany., Lang KS; Institute of Immunology, Medical Faculty, University Duisburg-Essen, 45122 Essen, Germany., van Kooyk Y; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands., Sancho D; Immunobiology Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain., Crocker PR; Division of Cell Signalling and Immunology, University of Dundee, Dundee, UK., den Haan JMM; Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands. Electronic address: j.denhaan@vumc.nl.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2018 Feb 06; Vol. 22 (6), pp. 1484-1495.
DOI:	10.1016/j.celrep.2018.01.021
Abstrakt:	Splenic CD169 + macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8 + T cell responses by antigens (Ags) bound by CD169 + macrophages. Upon Ag targeting to CD169 + macrophages, we show that BATF3-dependent CD8α + dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8 + T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8α + DCs and that Ag transfer to CD8α + DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8 + T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169 + macrophages and CD8α + DCs for the initiation of effective CD8 + T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8α + DCs. (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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