| Autor: |
Sunwoo IY; Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea., Hau NT; Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea., Ra CH; Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea., Jeong GT; Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea., Kim SK; Department of Biotechnology, Pukyong National University, Busan, 608-737, South Korea. skkim@pknu.ac.kr. |
| Abstrakt: |
The optimal conditions for acetone-butanol-ethanol (ABE) production were evaluated using waste seaweed from Gwangalli Beach, Busan, Korea. The waste seaweed had a fiber and carbohydrate, content of 48.34%; these are the main resources for ABE production. The optimal conditions for obtaining monosaccharides based on hyper thermal (HT) acid hydrolysis of waste seaweed were slurry contents of 8%, sulfuric acid concentration of 138 mM, and treatment time of 10 min. Enzymatic saccharification was performed using 16 unit/mL Viscozyme L, which showed the highest affinity (K m = 1.81 g/L). After pretreatment, 34.0 g/L monosaccharides were obtained. ABE fermentation was performed with single and sequential fermentation of Clostridium acetobutylicum and Clostridium tyrobutyricum; this was controlled for pH. A maximum ABE concentration of 12.5 g/L with Y ABE 0.37 was achieved using sequential fermentation with C. tyrobutyricum and C. acetobutylicum. Efficient ABE production from waste seaweed performed using pH-controlled culture broth and sequential cell culture. |
Full text from SpringerLink