| Autor: |
Joshi R; 1 Division of Critical Care Medicine and., Heinz A; 2 Martin Luther University, Halle-Wittenberg, Germany.; 3 University of Copenhagen, Copenhagen, Denmark., Fan Q; 1 Division of Critical Care Medicine and., Guo S; 4 Ionis Pharmaceuticals, La Jolla, California., Monia B; 4 Ionis Pharmaceuticals, La Jolla, California., Schmelzer CEH; 2 Martin Luther University, Halle-Wittenberg, Germany.; 5 Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle-Wittenberg, Germany., Weiss AS; 6 Charles Perkins Centre.; 7 Life and Environmental Sciences, and.; 8 Bosch Institute, University of Sydney, Sydney, Australia., Batie M; 9 Division of Clinical Engineering, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Parameshwaran H; 10 Department of Biomedical Engineering, Northeastern University, Boston, Massachusetts; and., Varisco BM; 1 Division of Critical Care Medicine and.; 11 Department of Pediatrics, University of Cincinnati, Cincinnati, Ohio. |
| Abstrakt: |
Alpha-1 antitrypsin (AAT) deficiency-related emphysema is the fourth leading indication for lung transplant. Chymotrypsin-like elastase 1 (Cela1) is a digestive protease that is expressed during lung development in association with regions of elastin remodeling, exhibits stretch-dependent expression during lung regeneration, and binds lung elastin in a stretch-dependent manner. AAT covalently neutralizes Cela1 in vitro. We sought to determine the role of Cela1 in postnatal lung physiology, whether it interacted with AAT in vivo, and to detect any effects it may have in the context of AAT deficiency. The lungs of Cela1 -/- mice had aberrant lung elastin structure and higher elastance as assessed with the flexiVent system. On the basis of in situ zymography with ex vivo lung stretch, Cela1 was solely responsible for stretch-inducible lung elastase activity. By mass spectrometry, Cela1 degraded mature elastin similarly to pancreatic elastase. Cela1 promoter and protein sequences were phylogenetically distinct in the placental mammal lineage, suggesting an adaptive role for lung-expressed Cela1 in this clade. A 6-week antisense oligonucleotide mouse model of AAT deficiency resulted in emphysema with increased Cela1 mRNA and reduction of approximately 70 kD Cela1, consistent with covalent binding of Cela1 by AAT. Cela1 -/- mice were completely protected against emphysema in this model. Cela1 was increased in human AAT-deficient emphysema. Cela1 is important in physiologic and pathologic stretch-dependent remodeling processes in the postnatal lung. AAT is an important regulator of this process. Our findings provide proof of concept for the development of anti-Cela1 therapies to prevent and/or treat AAT-deficient emphysema. |
