Association of SIRT-1 Gene Polymorphism and Vitamin D Level in Egyptian Patients With Rheumatoid Arthritis.

Autor:	Sabry D; Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt., Kaddafy SR; Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt., Abdelaziz AA; Department of Rheumatology and Rehabilitation, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Nassar AK; Department of Rheumatology and Rehabilitation, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Rayan MM; Department of Rheumatology and Rehabilitation, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Sadek SM; Department of internal medicine, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Abou-Elalla AA; Technology of Medical Laboratory, Faculty of Applied Medical Sciences, Misr University for Science and Technology, 6th of October City, Egypt.
Jazyk:	angličtina
Zdroj:	Journal of clinical medicine research [J Clin Med Res] 2018 Mar; Vol. 10 (3), pp. 189-195. Date of Electronic Publication: 2018 Jan 26.
DOI:	10.14740/jocmr3067e
Abstrakt:	Background: We investigated SIRT-1 genetic variant and its association with vitamin D level in Egyptian patients with rheumatoid arthritis (RA). Methods: Seventy Egyptian subjects were enrolled in our study and divided into two groups: RA group (n = 50 patients) and healthy control group (n = 20 subjects). Five milliliter blood sample was withdrawn from each subject followed by laboratory investigation and DNA extraction for SIRT-1 gene polymorphism assessment (rs7895833 A>G, rs7069102 C>G and rs2273773 C>T) and vitamin D level expression. Results: There was statistically significant difference between rheumatoid cases and controls with regard to vitamin D level with 88% of cases showing insufficient vitamin D versus all controls showing sufficient level. SIRT-1 different SNPs rs2273773, rs7895833and rs7069102 genotype frequencies were statistically significant in RA compared to control group (P = 0.001). There was no statistically significant difference between different genotypes of rs2273773, rs7895833 and rs7069102 with regard to vitamin D level. Conclusion: We concluded that there is a strong association between SIRT-1 polymorphism genotyping and RA. Vitamin D level was insufficient in Egyptian patients with RA.
Databáze:	MEDLINE
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