Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis.

Autor: Al-Farha AA; School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, South Australia, 5371, Australia. abdal-barahmednoori.al-farha@adelaide.edu.au.; Mosul Technical Institute, Technical Foundation, Northern Technical University, Mosul, 41000, Iraq. abdal-barahmednoori.al-farha@adelaide.edu.au., Petrovski K; School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, South Australia, 5371, Australia.; Australian Centre for Antimicrobial Resistance Ecology, The University of Adelaide, Adelaide, South Australia, 5000, Australia.; Davies Centre, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, South Australia, 5371, Australia., Jozani R; Department of Veterinary Clinical Sciences, University of Tabriz, Tabriz, 51666-14766, Iran., Hoare A; South East Vets, Mt Gambier, South Australia, 5290, Australia., Hemmatzadeh F; School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, South Australia, 5371, Australia.; Australian Centre for Antimicrobial Resistance Ecology, The University of Adelaide, Adelaide, South Australia, 5000, Australia.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2018 Feb 07; Vol. 11 (1), pp. 107. Date of Electronic Publication: 2018 Feb 07.
DOI: 10.1186/s13104-018-3223-y
Abstrakt: Objectives: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene.
Results: Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii, M. arginini, M. bovirhinis, M. bovis and uncultured Mycoplasma. Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection.
Databáze: MEDLINE
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