Changes in DNA Methylation from Age 18 to Pregnancy in Type 1, 2, and 17 T Helper and Regulatory T-Cells Pathway Genes.

Autor: Iqbal S; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. sabrina1.iqbal@gmail.com., Lockett GA; Human Development and Health, Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, UK. G.A.Lockett@soton.ac.uk., Holloway JW; Human Development and Health, Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, UK. J.W.Holloway@soton.ac.uk.; Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, UK. J.W.Holloway@soton.ac.uk., Arshad SH; Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, UK. S.H.Arshad@soton.ac.uk.; The David Hide Asthma and Allergy Research Centre, Newport PO30 5TG, UK. S.H.Arshad@soton.ac.uk., Zhang H; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. hzhang6@memphis.edu., Kaushal A; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. akaushl1@memphis.edu., Tetali SR; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. sabarinath5889@gmail.com., Mukherjee N; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. nmkhrjee@memphis.edu., Karmaus WJJ; Division of Epidemiology, Biostatistics and Environmental Health, School of Public Health, University of Memphis, Memphis, 301 Robison Hall, 3825 DeSoto Avenue Memphis, TN 38152, USA. karmaus1@memphis.edu.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2018 Feb 06; Vol. 19 (2). Date of Electronic Publication: 2018 Feb 06.
DOI: 10.3390/ijms19020477
Abstrakt: To succeed, pregnancies need to initiate immune biases towards T helper 2 (Th2) responses, yet little is known about what establishes this bias. Using the Illumina 450 K platform, we explored changes in DNA methylation (DNAm) of Th1, Th2, Th17, and regulatory T cell pathway genes before and during pregnancy. Female participants were recruited at birth (1989), and followed through age 18 years and their pregnancy (2011-2015). Peripheral blood DNAm was measured in 245 girls at 18 years; from among these girls, the DNAm of 54 women was repeatedly measured in the first (weeks 8-21, n = 39) and second (weeks 22-38, n = 35) halves of pregnancy, respectively. M-values (logit-transformed β-values of DNAm) were analyzed: First, with repeated measurement models, cytosine-phosphate-guanine sites (CpGs) of pathway genes in pregnancy and at age 18 (nonpregnant) were compared for changes ( p ≤ 0.05). Second, we tested how many of the 348 pathway-related CpGs changed compared to 10 randomly selected subsets of all other CpGs and compared to 10 randomly selected subsets of other CD4+-related CpGs (348 in each subset). Contrasted to the nonpregnant state, 27.7% of Th1-related CpGs changed in the first and 36.1% in the second half of pregnancy. Among the Th2 pathway CpGs, proportions of changes were 35.1% (first) and 33.8% (second half). The methylation changes suggest involvement of both Th1 and Th2 pathway CpGs in the immune bias during pregnancy. Changes in regulatory T cell and Th17 pathways need further exploration.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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