Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish.

Autor: Paravani EV; Laboratorio de Microscopia Aplicada a Estudios Moleculares y Celulares, Facultad de Ingeniería, Universidad Nacional de Entre Ríos (UNER), Ruta 11 Km 10, IBB-CONICET-UNER, Oro Verde, 3101, Entre Ríos, Argentina., Simoniello MF; Cátedra de Toxicología, Farmacología y Bioquímica Legal, FBCB-UNL, Ciudad Universitaria, CC242 Paraje El Pozo S/N, 3000, Santa Fe, Argentina., Poletta GL; Cátedra de Toxicología, Farmacología y Bioquímica Legal, FBCB-UNL, Ciudad Universitaria, CC242 Paraje El Pozo S/N, 3000, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), CABA, Argentina., Zolessi FR; Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Uruguay; Cell Biology of Neural Development Laboratory, Institut Pasteur de Montevideo, Uruguay., Casco VH; Laboratorio de Microscopia Aplicada a Estudios Moleculares y Celulares, Facultad de Ingeniería, Universidad Nacional de Entre Ríos (UNER), Ruta 11 Km 10, IBB-CONICET-UNER, Oro Verde, 3101, Entre Ríos, Argentina. Electronic address: vcasco@ingenieria.uner.edu.ar.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2018 Feb; Vol. 826, pp. 25-32. Date of Electronic Publication: 2017 Dec 27.
DOI: 10.1016/j.mrgentox.2017.12.010
Abstrakt: Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, a marker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker, was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxide-induced DNA damage, showing a dose-dependent response. We observed a positive comet assay response to CM that was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and cat mRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induce apoptosis in retinal cells.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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