Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague-Dawley rats.
Autor: | Jensen VS; 1Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg, Denmark.; 2Insulin Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Hvid H; 2Insulin Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Damgaard J; 2Insulin Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Nygaard H; 2Insulin Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Ingvorsen C; 3Histology and Imaging, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Wulff EM; 4Obesity and Diabetes Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark., Lykkesfeldt J; 1Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg, Denmark., Fledelius C; 2Insulin Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Maaloev, Denmark. |
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Jazyk: | angličtina |
Zdroj: | Diabetology & metabolic syndrome [Diabetol Metab Syndr] 2018 Jan 24; Vol. 10, pp. 4. Date of Electronic Publication: 2018 Jan 24 (Print Publication: 2018). |
DOI: | 10.1186/s13098-018-0307-8 |
Abstrakt: | Background: In humans and animal models, excessive intake of dietary fat, fructose and cholesterol has been linked to the development of non-alcoholic fatty liver disease (NAFLD). However, the individual roles of the dietary components remain unclear. To investigate this further, we compared the effects of a high-fat diet, a high-fructose diet and a combination diet with added cholesterol on the development of NAFLD in rats. Methods: Forty male Sprague-Dawley rats were randomized into four groups receiving either a control-diet (Control: 10% fat); a high-fat diet (HFD: 60% fat, 20% carbohydrate), a high-fructose diet [HFr: 10% fat, 70% carbohydrate (mainly fructose)] or a high-fat/high-fructose/high-cholesterol-diet (NASH: 40% fat, 40% carbohydrate (mainly fructose), 2% cholesterol) for 16 weeks. Results: After 16 weeks, liver histology revealed extensive steatosis and inflammation in both NASH- and HFD-fed rats, while hepatic changes in HFr-rats were much more subtle. These findings were corroborated by significantly elevated hepatic triglyceride content in both NASH- ( p < 0.01) and HFD-fed rats ( p < 0.0001), elevated hepatic cholesterol levels in NASH-fed rats ( p < 0.0001), but no changes in HFr-fed rats, compared to Control. On the contrary, only HFr-fed rats developed dyslipidemia as characterized by higher levels of plasma triglycerides compared to all other groups ( p < 0.0001). Hepatic dysfunction and inflammation was confirmed in HFD-fed rats by elevated levels of hepatic MCP-1 ( p < 0.0001), TNF-alpha ( p < 0.001) and plasma β-hydroxybutyrate ( p < 0.0001), and in NASH-fed rats by elevated levels of hepatic MCP-1 ( p < 0.01), increased hepatic macrophage infiltration ( p < 0.001), and higher plasma levels of alanine aminotransferase ( p < 0.0001) aspartate aminotransferase ( p < 0.05), haptoglobin ( p < 0.001) and TIMP-1 ( p < 0.01) compared to Control. Conclusion: These findings show that dietary fat and cholesterol are the primary drivers of NAFLD development and progression in rats, while fructose mostly exerts its effect on the circulating lipid pool. |
Databáze: | MEDLINE |
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