Hybrid PCL/CaCO 3 scaffolds with capabilities of carrying biologically active molecules: Synthesis, loading and in vivo applications.

Autor: Saveleva MS; Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Astrakhanskaya 83, Saratov 410012, Russia; Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent 9000, Belgium. Electronic address: Mariia.Saveleva@UGent.be., Ivanov AN; Research Institute of Traumatology, Orthopaedics and Neurosurgery, Saratov State Medical University, Chernyshevskogo 148, Saratov 410002, Russia; Department of Histology, Saratov State Medical University, B. Kazachya 112, Saratov 410012, Russia., Kurtukova MO; Department of Histology, Saratov State Medical University, B. Kazachya 112, Saratov 410012, Russia., Atkin VS; Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Astrakhanskaya 83, Saratov 410012, Russia., Ivanova AG; FSRC Crystallography and Photonics RAS, Leninskiy prospect 59, Moscow 119333, Russia., Lyubun GP; Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Astrakhanskaya 83, Saratov 410012, Russia., Martyukova AV; Department of Histology, Saratov State Medical University, B. Kazachya 112, Saratov 410012, Russia., Cherevko EI; Department of Histology, Saratov State Medical University, B. Kazachya 112, Saratov 410012, Russia., Sargsyan AK; Department of Histology, Saratov State Medical University, B. Kazachya 112, Saratov 410012, Russia., Fedonnikov AS; Research Institute of Traumatology, Orthopaedics and Neurosurgery, Saratov State Medical University, Chernyshevskogo 148, Saratov 410002, Russia., Norkin IA; Research Institute of Traumatology, Orthopaedics and Neurosurgery, Saratov State Medical University, Chernyshevskogo 148, Saratov 410002, Russia., Skirtach AG; Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent 9000, Belgium., Gorin DA; Skoltech center of Photonics & Quantum Materials, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow 143026, Russia; Educational Research Institute of Nanostructures and Biosystems, Saratov State University, Astrakhanskaya 83, Saratov 410012, Russia., Parakhonskiy BV; Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent 9000, Belgium; FSRC Crystallography and Photonics RAS, Leninskiy prospect 59, Moscow 119333, Russia. Electronic address: Bogdan.Parakhonskiy@UGent.be.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2018 Apr 01; Vol. 85, pp. 57-67. Date of Electronic Publication: 2017 Dec 15.
DOI: 10.1016/j.msec.2017.12.019
Abstrakt: Designing advanced biomaterials for tissue regeneration with drug delivery and release functionalities remains a challenge in regenerative medicine. In this research, we have developed novel composite scaffolds based on polymeric polycaprolactone fibers coated with porous calcium carbonate structures (PCL/CaCO 3 ) for tissue engineering and have shown their drug delivery and release in rats. In vivo biocompatibility tests of PCL/CaCO 3 scaffolds were complemented with in vivo drug release study, where tannic acid (TA) was used as a model drug. Release of TA from the scaffolds was realized by recrystallization of the porous vaterite phase of calcium carbonate into the crystalline calcite. Cell colonization and tissue vascularization as well as transplantability of developed PCL/CaCO 3 +TA scaffolds were observed. Detailed study of scaffold transformations during 21-day implantation period was followed by scanning electron microscopy and X-ray diffraction studies before and after in vivo implantation. The presented results demonstrate that PCL/CaCO 3 scaffolds are attractive candidates for implants in bone regeneration and tissue engineering with a possibility of loading biologically active molecules and controlled release.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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