| Autor: |
de Oliveira JK; Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá, 85040-080 Guarapuava, Brazil., Ronik DFV; Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá, 85040-080 Guarapuava, Brazil., Ascari J; Department of Biological Sciences, Universidade Tecnológica Federal do Paraná, Rua Cerejeiras S/N, 85892-000 Santa Helena, PR, Brazil., Mainardes RM; Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá, 85040-080 Guarapuava, Brazil., Khalil NM; Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Rua Simeão Camargo Varela de Sá, 85040-080 Guarapuava, Brazil. |
| Abstrakt: |
In this study, we developed and validated a fast, specific, sensitive, precise and stability-indicating high performance liquid chromatography (HPLC) method to determine the drug apocynin in bovine serum albumin (BSA) nanoparticles. Chromatographic analyses were performed on an RP C 18 column and using a photodiode array detector at a wavelength of 276 nm. Mobile phase consisted of a mixture of acetonitrile and 1% acetic acid (60:40, v/v), and it was eluted isocratically at a flow rate of 0.8 mL/min. The retention time of apocynin chromatographic peak was 1.65 min. The method was linear, precise, accurate and specific in the range of 5-100 μg/mL. The intra- and inter-day precisions presented relative standard deviation (RSD) values lower than 2%. The method was robust regarding changes in mobile phase proportion, but not for flow rate. Limits of detection and quantitation were 78 ng/mL and 238 ng/mL, respectively. Apocynin was exposed to acid and alkali hydrolysis, oxidation and visible light. The drug suffered mild degradation under acid and oxidation conditions and great degradation under alkali conditions. Light exposure did not degrade the drug. The method was successfully applied to determine the encapsulation efficiency of apocynin in BSA nanoparticles. |
