Bioanalytical method development and validation of milnacipran in rat plasma by LC-MS/MS detection and its application to a pharmacokinetic study.
| Autor: | Kanala K; Jawaharlal Nehru Technological University Anantapur, Andhrapradesh 515002, India.; Ratnam Institute of Pharmacy, Pidatapolur, Muthukur, Nellore, Andhrapradesh 524346, India., T Hwisa N; Faculty of Pharmacy,University of Al-Zawia, 13, Libya., Chandu BR; Faculty of Pharmacy,University of Al-Zawia, 13, Libya., Katakam P; Faculty of Pharmacy,University of Al-Zawia, 13, Libya., Khagga M; Jawaharlal Nehru Technological University Hyderabad, Andhrapradesh 500072, India., Challa BR; Nirmala College of Pharmacy, Madras road, Kadapa, Andhrapradesh 516002, India., Khagga B; Center for Pharmaceutical Sciences Department, J. N. T. University, Kukatpally, Hyderabad, Andhra Pradesh 500072, India. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of pharmaceutical analysis [J Pharm Anal] 2013 Dec; Vol. 3 (6), pp. 481-488. Date of Electronic Publication: 2013 Apr 29. |
| DOI: | 10.1016/j.jpha.2013.03.009 |
| Abstrakt: | A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of milnacipran (MC) in rat plasma by using the liquid-liquid extraction method. Milnacipran-d10 (MCD10) was used as an internal standard (IS). Chromatographic separation was achieved on Zorbax SB-CN (4.6 mm×75 mm, 3.5 µm) column with an isocratic mobile phase composed of 10 mM ammonium acetate (pH 4.0) and methanol in the ratio of 25:75(v/v), at a flow-rate of 0.7 mL/min. MC and MCD10 were detected with proton adducts at m/z 247.2→230.3 and m/z 257.2→240.4 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated over a linear concentration range of 1.00-400.00 ng/mL with a correlation coefficient ( r 2 )≥0.9850. This method demonstrated intra- and inter-day precision within 5.40-10.85% and 4.40-8.29% and accuracy within 97.00-104.20% and 101.64-106.23%. MC was found to be stable throughout three freeze-thaw cycles, bench top and postoperative stability studies. This method was successfully applied to a pharmacokinetic study of rats through i.v. administration. |
| Databáze: | MEDLINE |
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