Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study.

Autor: Kumari Karra V; University College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500085, India., Rao Pilli N; University College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500085, India., Kumar Inamadugu J; Analytical and Environmental Chemistry Division, Department of Chemistry, Sri Venkateswara University, Tirupati-517502, India., Seshagiri Rao JVLN; College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical analysis [J Pharm Anal] 2012 Jun; Vol. 2 (3), pp. 167-173. Date of Electronic Publication: 2012 Jan 28.
DOI: 10.1016/j.jpha.2012.01.002
Abstrakt: A simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma. Irbesartan was used as an internal standard. The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 μm polymeric sorbent. The reconstituted samples were chromatographed on a C 18 column by using a 80:20 (v/v) mixture of acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.8 mL/min. The calibration curves obtained were linear ( r ≥0.99) over the concentration range of 15-3000 ng/mL for pioglitazone and 5-608 ng/mL for candesartan. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. A run time of 2.7 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies.
Databáze: MEDLINE
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