Parallel Effects of Methamphetamine on Anxiety and CCL3 in Humans and a Genetic Mouse Model of High Methamphetamine Intake.

Autor:	Huckans M; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA.; Department of Psychiatry, Oregon Health and Science University, Portland, Oregon, USA.; Mental Health and Clinical Neurosciences Division, VA Portland Health Care System, Portland, Oregon, USA.; Methamphetamine Abuse Research Center, Oregon Health and Science University, Portland, Oregon, USA., Wilhelm CJ; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA.; Department of Psychiatry, Oregon Health and Science University, Portland, Oregon, USA., Phillips TJ; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA.; Methamphetamine Abuse Research Center, Oregon Health and Science University, Portland, Oregon, USA.; Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, Oregon, USA., Huang ET; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA.; Methamphetamine Abuse Research Center, Oregon Health and Science University, Portland, Oregon, USA., Hudson R; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA., Loftis JM; Research and Development Service, VA Portland Health Care System, Portland, Oregon, USA.; Department of Psychiatry, Oregon Health and Science University, Portland, Oregon, USA.; Methamphetamine Abuse Research Center, Oregon Health and Science University, Portland, Oregon, USA.
Jazyk:	angličtina
Zdroj:	Neuropsychobiology [Neuropsychobiology] 2017; Vol. 75 (4), pp. 169-177. Date of Electronic Publication: 2018 Jan 18.
DOI:	10.1159/000485129
Abstrakt:	Background: Methamphetamine (MA) abuse causes immune dysfunction and neuropsychiatric impairment. The mechanisms underlying these deficits remain unidentified. Methods: The effects of MA on anxiety-like behavior and immune function were investigated in mice selectively bred to voluntarily consume high amounts of MA [i.e., MA high drinking (MAHDR) mice]. MA (or saline) was administered to mice using a chronic (14-day), binge-like model. Performance in the elevated zero maze (EZM) was determined 5 days after the last MA dose to examine anxiety-like behavior. Cytokine and chemokine expressions were measured in the hippocampus using quantitative polymerase chain reaction (qPCR). Human studies were also conducted to evaluate symptoms of anxiety using the General Anxiety Disorder-7 Scale in adults with and without a history of MA dependence. Plasma samples collected from human research participants were used for confirmatory analysis of murine qPCR results using an enzyme-linked immunosorbent assay. Results: During early remission from MA, MAHDR mice exhibited increased anxiety-like behavior on the EZM and reduced expression of chemokine (C-C motif) ligand 3 (ccl3) in the hippocampus relative to saline-treated mice. Human adults actively dependent on MA and those in early remission had elevated symptoms of anxiety as well as reductions in plasma levels of CCL3, relative to adults with no history of MA abuse. Conclusions: The results highlight the complex effects of MA on immune and behavioral function and suggest that alterations in CCL3 signaling may contribute to the mood impairments observed during remission from MA addiction. (© 2018 S. Karger AG, Basel.)
Databáze:	MEDLINE
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