Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace.

Autor:	Zhou YJ; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Yang Q; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Zhong XJ; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Tang HP; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Deng M; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Ma J; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Qi PF; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Wang JR; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Chen GY; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Liu YX; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Lu ZX; b Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada., Li W; c College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Lan XJ; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Wei YM; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Zheng YL; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Jiang QT; a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Jazyk:	angličtina
Zdroj:	Genome [Genome] 2018 Mar; Vol. 61 (3), pp. 201-208. Date of Electronic Publication: 2018 Feb 05.
DOI:	10.1139/gen-2017-0246
Abstrakt:	We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE. The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119). An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron-exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring. Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs. Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II. In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon. The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content. As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.
Databáze:	MEDLINE
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