| Autor: |
Stadnicka-Michalak J; Eawag , Überlandstrasse 133 , 8600 Dübendorf , Switzerland.; School of Architecture, Civil and Environmental Engineering , EPF Lausanne , 1015 Lausanne , Switzerland., Weiss FT; Eawag , Überlandstrasse 133 , 8600 Dübendorf , Switzerland.; Institute of Biogeochemistry and Pollutant Dynamics , ETH Zürich , 8092 Zürich , Switzerland., Fischer M; Eawag , Überlandstrasse 133 , 8600 Dübendorf , Switzerland., Tanneberger K; Eawag , Überlandstrasse 133 , 8600 Dübendorf , Switzerland.; Ecosens AG, 8304 Wallisellen , Switzerland., Schirmer K; Eawag , Überlandstrasse 133 , 8600 Dübendorf , Switzerland.; School of Architecture, Civil and Environmental Engineering , EPF Lausanne , 1015 Lausanne , Switzerland.; Institute of Biogeochemistry and Pollutant Dynamics , ETH Zürich , 8092 Zürich , Switzerland. |
| Abstrakt: |
Permanent fish cell lines constitute a promising complement or substitute for fish in the environmental risk assessment of chemicals. We demonstrate the potential of a set of cell lines originating from rainbow trout ( Oncorhynchus mykiss) to aid in the prediction of chemical bioaccumulation in fish, using benzo[ a]pyrene (BaP) as a model chemical. We selected three cell lines from different tissues to more fully account for whole-body biotransformation in vivo: the RTL-W1 cell line, representing the liver as major site of biotransformation, and the RTgill-W1 (gill) and RTgutGC (intestine) cell lines, as important environment-organism interfaces, which likely influence chemical uptake. All three cell lines were found to effectively biotransform BaP. However, rates of in vitro clearance differed, with the RTL-W1 cell line being most efficient, followed by RTgutGC. Co-exposures with α-naphthoflavone as potent inhibitor of biotransformation, assessment of CYP1A catalytic activity, and the progression of cellular toxicity upon prolonged BaP exposure revealed that BaP is handled differently in the RTgill-W1 compared to the other two cell lines. Application of the cell-line-derived in vitro clearance rates into a physiology-based toxicokinetic model predicted a BaP bioconcentration factor (BCF) of 909-1057 compared to 920 reported for rainbow trout in vivo. |
