Usefulness of FC-TRIPLEX Chagas/Leish IgG1 as confirmatory assay for non-negative results in blood bank screening of Chagas disease.

Autor: Campos FMF; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil; Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Repoles LC; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil., de Araújo FF; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil; Programa de Pós graduação em Sanidade e Produção Animal nos Trópicos, Universidade de Uberaba, Uberaba, MG, Brazil., Peruhype-Magalhães V; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil., Xavier MAP; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil., Sabino EC; Fundação Pró-Sangue, São Paulo, SP, Brazil; Instituto de Medicina Tropical, Universidade de São Paulo - USP, São Paulo, SP, Brazil., de Freitas Carneiro Proietti AB; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - HEMOMINAS, Belo Horizonte, MG, Brazil., Andrade MC; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil; Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil., Teixeira-Carvalho A; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil., Martins-Filho OA; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil. Electronic address: oamfilho@minas.fiocruz.br., Gontijo CMF; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil.
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2018 Apr; Vol. 455, pp. 34-40. Date of Electronic Publication: 2018 Jan 31.
DOI: 10.1016/j.jim.2018.01.006
Abstrakt: A relevant issue in Chagas disease serological diagnosis regards the requirement of using several confirmatory methods to elucidate the status of non-negative results from blood bank screening. The development of a single reliable method may potentially contribute to distinguish true and false positive results. Our aim was to evaluate the performance of the multiplexed flow-cytometry anti-T. cruzi/Leishmania IgG1 serology/(FC-TRIPLEX Chagas/Leish IgG1) with three conventional confirmatory criteria (ELISA-EIA, Immunofluorescence assay-IIF and EIA/IIF consensus criterion) to define the final status of samples with actual/previous non-negative results during anti-T. cruzi ELISA-screening in blood banks. Apart from inconclusive results, the FC-TRIPLEX presented a weak agreement index with EIA, while a strong agreement was observed when either IIF or EIA/IIF consensus criteria were applied. Discriminant analysis and Spearman's correlation further corroborates the agreement scores. ROC curve analysis showed that FC-TRIPLEX performance indexes were higher when IIF and EIA/IIF consensus were used as a confirmatory criterion. Logistic regression analysis further demonstrated that the probability of FC-TRIPLEX to yield positive results was higher for inconclusive results from IIF and EIA/IIF consensus. Machine learning tools illustrated the high level of categorical agreement between FC-TRIPLEX versus IIF or EIA/IIF consensus. Together, these findings demonstrated the usefulness of FC-TRIPLEX as a tool to elucidate the status of non-negative results in blood bank screening of Chagas disease.
(Copyright © 2018. Published by Elsevier B.V.)
Databáze: MEDLINE
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