Effect of human, dentin, albumin and lipopolysaccharide on the antibacteerial activity of endodontic activity of endodontic irrigants.

Autor:	Quintana RM; Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Jardine AP; Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Montagner F; Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Fatturi Parolo CC; Department of Social and Preventive Dentistry, Faculty of Dentstry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Morgental RD; Department of Stomatology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Poli Kopper PM; Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of conservative dentistry : JCD [J Conserv Dent] 2017 Sep-Oct; Vol. 20 (5), pp. 341-345.
DOI:	10.4103/JCD.JCD_129_16
Abstrakt:	Context: Human dentin powder (HD), bovine serum albumin (BSA) and endotoxin (LPS) may affect the antimicrobial activity of irrigating solutions. Aim: To evaluate the inhibitory effect of HD powder, BSA, and LPS on the antibacterial activity of 0.5%, 1%, 2.5%, and 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) gel, BioPure mixture of tetracycline, citric acid, and detergent (MTAD), and QMix. Methods: The direct contact test against Enterococcus faecalis (ATCC 29212) for 2-min, 30-min and 6-h was used. Sterile pyrogen-free water was the negative control. After experimental periods, a neutralizing agent was used. Colony-forming units were determined by 10-fold serial dilutions and culture on agar plates. Data were analyzed by Kruskal-Wallis and Dunn's test (α = 5%). Results: In the absence of inhibitors, all irrigants eliminated E. faecalis . In contact with HD, all solutions eliminated E. faecalis within 2-min, with the exception of MTAD. In the presence of BSA, only 5% NaOCl killed E. faecalis within 2-min. LPS did not affect the antibacterial effect of any irrigant. At 30-min and at 6-h, all substances eliminated E. faecalis . Conclusions: In the presence of albumin, irrigants needed >2-min to eliminate E. faecalis , except for 5% NaOCl. The same was observed in the presence of dentin when E. faecalis was exposed to MTAD. Competing Interests: There are no conflicts of interest.
Databáze:	MEDLINE
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