| Autor: |
Khodanovich MY; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Kisel AA; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Akulov AE; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation.; 2 Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Atochin DN; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation.; 3 Cardiovascular Research Center, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.; 4 RASA Center in Tomsk, Tomsk Polytechnic University, Tomsk, Russian Federation., Kudabaeva MS; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Glazacheva VY; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Svetlik MV; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Medvednikova YA; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation., Mustafina LR; 5 Department of Histology, Embryology and Cytology, Siberian State Medical University, Tomsk, Russian Federation., Yarnykh VL; 1 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation.; 6 Department of Radiology, University of Washington, Seattle, WA, USA. |
| Abstrakt: |
A recent MRI method, fast macromolecular proton fraction (MPF) mapping, was used to quantify demyelination in the transient middle cerebral artery occlusion (MCAO) rat stroke model. MPF and other quantitative MRI parameters (T 1 , T 2 , proton density, and apparent diffusion coefficient) were compared with histological and immunohistochemical markers of demyelination (Luxol Fast Blue stain, (LFB)), neuronal loss (NeuN immunofluorescence), axonal loss (Bielschowsky stain), and inflammation (Iba1 immunofluorescence) in three animal groups ( n = 5 per group) on the 1st, 3rd, and 10th day after MCAO. MPF and LFB optical density (OD) were significantly reduced in the ischemic lesion on all days after MCAO relative to the symmetrical regions of the contralateral hemisphere. Percentage changes in MPF and LFB OD in the ischemic lesion relative to the contralateral hemisphere significantly differed on the first day only. Percentage changes in LFB OD and MPF were strongly correlated (R = 0.81, P < 0.001) and did not correlate with other MRI parameters. MPF also did not correlate with other histological variables. Addition of T 2 into multivariate regression further improved agreement between MPF and LFB OD (R = 0.89, P < 0.001) due to correction of the edema effect. This study provides histological validation of MPF as an imaging biomarker of demyelination in ischemic stroke. |
