| Autor: |
Babbar M; Department of Pharmacology, SUNY Upstate Medical University, Syracuse, New York, USA., Huang Y; Department of Pharmacology, SUNY Upstate Medical University, Syracuse, New York, USA., An J; Department of Pharmacology, SUNY Upstate Medical University, Syracuse, New York, USA.; Gulfstream Diagnostics-Genomics, Dallas, Texas, USA., Landas SK; Department of Pathology, SUNY Upstate Medical University, Syracuse, New York, USA., Sheikh MS; Department of Pharmacology, SUNY Upstate Medical University, Syracuse, New York, USA. sheikhm@upstate.edu. |
| Abstrakt: |
A better understanding of the link between cellular metabolism and tumorigenesis is needed. Here, we report characterization of a novel protein named coiled-coil helix tumor and metabolism 1 (CHTM1). We have found that CHTM1 is associated with cancer and cellular metabolism. CHTM1 localizes to mitochondria and cytosol, and its deficiency in cancer cells results in decreased mitochondrial oxygen consumption and ATP levels as well as oxidative stress indicating mitochondrial dysfunction. CHTM1-deficient cancer cells display poor growth under glucose/glutamine-deprived conditions, whereas cells expressing increased levels of exogenous CHTM1 exhibit enhanced proliferation and survival under similar conditions. CHTM1 deficiency also leads to defects in lipid metabolism resulting in fatty acid accumulation, which explains poor growth of CHTM1-deficient cells under glucose/glutamine deprivation since nutrient deprivation increases dependency on lipids for energy generation. We also demonstrate that CHTM1 mediates its effect via the PKC, CREB, and PGC-1alpha signaling axis, and cytosolic accumulation of CHTM1 during nutrient deprivation appears to be important for its effect on cellular signaling events. Furthermore, analyses of tissue specimens from 71 breast and 97 colon cancer patients show CHTM1 expression to be upregulated in the majority of tumor specimens representing these malignancies. Collectively, our findings are highly significant because CHTM1 is a novel metabolic marker that is important for the growth of tumorigenic cells under limiting nutrient supplies and thus, links cellular metabolism and tumorigenesis. |
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