| Autor: |
Adav SS; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore. ssadav@ntu.edu.sg., Subbaiaih RS; Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore., Kerk SK; Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore., Lee AY; Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore., Lai HY; School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore.; Nanyang Environment and Water Research Institute, (Environmental Chemistry and Materials Centre), Interdisciplinary Graduate School, Nanyang Technological University, Singapore, Singapore., Ng KW; School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore.; Nanyang Environment and Water Research Institute, (Environmental Chemistry and Materials Centre), Interdisciplinary Graduate School, Nanyang Technological University, Singapore, Singapore.; Skin Research Institute of Singapore, Singapore, Singapore., Sze SK; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore., Schmidtchen A; Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore.; Division of Dermatology, Department of Clinical Sciences, Lund University, Lund, Sweden.; Wound Healing Center, Bispebjerg Hospital, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark. |
| Abstrakt: |
Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed a significant deamidation of keratin type I and II, and KAPs. The hair shafts were found to contain several types of histones, which are well known to exert antimicrobial activity. Analysis of the hair proteome, particularly its composition, protein abundances, deamidated hair proteins, and modification sites, may offer a novel approach to explore potential biomarkers of hair health quality, hair diseases, and aging. |
