Use of counter-current chromatography as a selective extractor for the diterpenequinone 7α-hydroxyroyleanone from Tetradenia riparia.

Autor: Milato JV; Universidade Federal do Rio de Janeiro, Instituto de Pesquisas de Produtos Naturais, CCS, Bl. H, Ilha do Fundão, Rio de Janeiro, RJ, Brazil., Silva RSF; Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Campus Rio de Janeiro, Maracanã, Rio de Janeiro, RJ, Brazil., Figueiredo FS; Universidade Federal do Rio de Janeiro, Instituto de Pesquisas de Produtos Naturais, CCS, Bl. H, Ilha do Fundão, Rio de Janeiro, RJ, Brazil., Azevedo DA; Universidade Federal do Rio de Janeiro, Instituto de Química, LAGOA-LADETEC, Ilha do Fundão, Rio de Janeiro, RJ, Brazil., Ribeiro CAB; Universidade Federal do Rio de Janeiro, Instituto de Química, LAGOA-LADETEC, Ilha do Fundão, Rio de Janeiro, RJ, Brazil., Leitão GG; Universidade Federal do Rio de Janeiro, Instituto de Pesquisas de Produtos Naturais, CCS, Bl. H, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. Electronic address: ggleitao@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2018 Feb 16; Vol. 1537, pp. 135-140. Date of Electronic Publication: 2018 Jan 09.
DOI: 10.1016/j.chroma.2018.01.020
Abstrakt: A solvent system was developed for selective isolation by high-speed counter-current chromatography (HSCCC) of the benzoquinone 7α-hydroxyroileanone, 1, a bioactive diterpene from a dichloromethane extract of Tetradenia riparia leaves. Several solvent systems were initially studied, including hexane-ethyl acetate-methanol-water in several ratios, hexane-acetone-methanol-water, hexane-ethanol-water and hexane-acetonitrile-methanol, which gave recovery rates for the target compound between 13.4 and 35.9%. The new solvent system hexane-5% aqueous Na 2 CO 3 (1:1) was developed based on the chemical ionization reaction of the benzoquinone hydroxyl group in the basic pH of the carbonate solution, prompted by the extraction procedure used for the extraction of lapachol (a natural naphtoquinone) from a Tabebuia species wood. By using the HSCCC chromatograph as a liquid-liquid extractor with the above mentioned solvent system the recovery rate of 1 increased to 81.8%, affording the quinone with 97% purity.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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