Genome-wide association analysis and QTL mapping reveal the genetic control of cadmium accumulation in maize leaf.

Autor: Zhao X; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Luo L; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Cao Y; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Liu Y; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Li Y; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Wu W; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Lan Y; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Jiang Y; Department of Agronomy, Purdue University, West Lafayette, 47906, USA., Gao S; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Zhang Z; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Shen Y; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China., Pan G; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. pangt@sicau.edu.cn., Lin H; Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. linhj521@gmail.com.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2018 Jan 25; Vol. 19 (1), pp. 91. Date of Electronic Publication: 2018 Jan 25.
DOI: 10.1186/s12864-017-4395-x
Abstrakt: Background: Accumulation of cadmium (Cd) in maize (Zea mays L.) poses a significant risk to human health as it is ingested via the food chain. A genome-wide association study (GWAS) was conducted in a population of 269 maize accessions with 43,737 single nucleotide polymorphisms (SNPs) to identify candidate genes and favorable alleles for controlling Cd accumulation in maize.
Results: When grown in contaminated soil, accessions varied significantly in leaf Cd concentration at both the seeding and maturing stages with phenotypic variation and the coefficient of variation all above 48%. The co-localized region between SYN27837 (147,034,650 bp) and SYN36598 (168,551,327 bp) on chromosome 2 was associated with leaf Cd under three soil conditions varying in Cd content in 2015 and 2016. The significant SNP (SYN25051) at position 161,275,547 could explained 27.1% of the phenotype variation. Through QTL mapping using the IBMSyn10 double haploid (DH) population, we validated the existence of a major QTL identified by GWAS; qLCd2 could explain the 39.8% average phenotype variation across the experiments. Expression of GRMZM2G175576 encoding a cadmium/zinc-transporting ATPase underlying the QTL was significantly increased in roots, stems and leaves of B73, a low Cd accumulation line in response to Cd stress.
Conclusions: Our findings provide new insights into the genetic control of Cd accumulation and could aid rapid development of maize genotypes with low-Cd accumulation by manipulation of the favorable alleles.
Databáze: MEDLINE
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