Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica.

Autor: Andrade ACDSP; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Arantes TS; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Rodrigues RAL; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Machado TB; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Dornas FP; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Landell MF; Laboratório de Diversidade Molecular, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, Brazil., Furst C; Departamento de Patologia, Universidade Federal do Espírito Santo, Maruípe, Brazil., Borges LGA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Instituto do Petróleo e dos Recursos Naturais (IPR), Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil., Dutra LAL; Department of Biological and Environmental Sciences, University of Jyvaskyla, Jyvaskyla, Finland., Almeida G; Department of Biological and Environmental Sciences, University of Jyvaskyla, Jyvaskyla, Finland., Trindade GS; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Bergier I; Embrapa Pantanal, Corumbá, Brazil., Abrahão W; Universidade Federal de Viçosa, Viçosa, Brazil., Borges IA; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Cortines JR; Departamento de Virologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira DB; Faculdade de Medicina, Universidade Federal do dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazil., Kroon EG; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Abrahão JS; Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. jonatas.abrahao@gmail.com.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2018 Jan 24; Vol. 15 (1), pp. 22. Date of Electronic Publication: 2018 Jan 24.
DOI: 10.1186/s12985-018-0930-x
Abstrakt: Background: Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica.
Methods: Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission).
Results: A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far).
Conclusions: Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature.
Databáze: MEDLINE
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