Molecular epidemiology of the endemic multiresistance plasmid pSI54/04 of Salmonella Infantis in broiler and human population in Hungary.

Autor: Szmolka A; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary. Electronic address: szmolka.annamaria@agrar.mta.hu., Szabó M; NARIC Agricultural Biotechnology Center, Szent-Györgyi Albert u. 4, 2100 Gödöllő, Hungary., Kiss J; NARIC Agricultural Biotechnology Center, Szent-Györgyi Albert u. 4, 2100 Gödöllő, Hungary., Pászti J; National Center for Epidemiology, Albert Flórián út 2-6, 1097 Budapest, Hungary., Adrián E; National Food Chain Safety Office, Food and Feed Safety Directorate, Budapest, Hungary., Olasz F; NARIC Agricultural Biotechnology Center, Szent-Györgyi Albert u. 4, 2100 Gödöllő, Hungary., Nagy B; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary.
Jazyk: angličtina
Zdroj: Food microbiology [Food Microbiol] 2018 May; Vol. 71, pp. 25-31. Date of Electronic Publication: 2017 Mar 16.
DOI: 10.1016/j.fm.2017.03.011
Abstrakt: Salmonella Infantis (SI) became endemic in Hungary where the PFGE cluster B, characterized by a large multiresistance (MDR) plasmid emerged among broilers leading to an increased occurrence in humans. We hypothesized that this plasmid (pSI54/04) assisted dissemination of SI. Indeed, Nal-Sul-Tet phenotypes carrying pSI54/04 occurred increasingly between 2011 and 2013 among SI isolates from broilers and humans. Characterization of pSI54/04 based on genome sequence data of the MDR strain SI54/04 indicated a size of ∼277 kb and a high sequence similarity with the megaplasmid pESI of SI predominant in Israel. Molecular characterization of 78 representative broiler and human isolates detected the prototype plasmid pSI54/04 and its variants together with novel plasmid associations within the emerging cluster B. To test in vitro and in vivo pathogenicity of pSI54/04 we produced plasmidic transconjugant of the plasmid-free pre-emergent strain SI69/94. This parental strain and its transconjugant have been tested on chicken embryo fibroblasts (CEFs) and in orally infected day old chicks. The uptake of pSI54/04 did not increase the pathogenicity of the strain SI69/94 in these systems. Thus, dissemination of SI in poultry could be assisted by antimicrobial resistance rather than by virulence modules of the endemic plasmid pSI54/04 in Hungary.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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