Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China.

Autor: Liu BJ; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China., Zuo YZ; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China.; College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China., Gu WY; Animal Diseases Control Center of Hebei, Shijiazhuang, China., Luo SX; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China., Shi QK; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China., Hou LS; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China., Zhong F; College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China., Fan JH; College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2018 Jun; Vol. 65 (3), pp. 874-882. Date of Electronic Publication: 2018 Jan 24.
DOI: 10.1111/tbed.12821
Abstrakt: Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB-BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB-BD strain was sequenced and analysed. Genomic analysis showed that the HB-BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains.
(© 2018 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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