| Autor: |
Eggers LF; Division of Bioanalytical Chemistry, Research Center Borstel, Borstel, 23845, Germany., Schwudke D; Division of Bioanalytical Chemistry, Research Center Borstel, Borstel, 23845, Germany. dschwudke@fz-borstel.de. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1730, pp. 163-174. |
| DOI: |
10.1007/978-1-4939-7592-1_12 |
| Abstrakt: |
Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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